• Cover the slide with 10 drops of pure May-Grunwald stain and allow it to react for 3 minutes.
• Add drops of neutralized water to the slide and agitate gently to homogenize. 
  Allow it to react for 1 minute.
• Pour off the mixture. Do not wash up. 
• Cover the slide with a diluted Giemsa stain prepared extemporaneously with neutralized water:
     - Giemsa stain diluted at 1/10 for 10 minutes
     - Giemsa stain diluted at 1/20 for 20 minutes for better contrast 
  
• Wash up with neutralized water. 
• Wipe the slide on the reverse side of the smear.
• Dry: drying oven, hair drier, electric fan.  

ATTENTION
THE MAY-GRUNWALD STAIN IS NOT STABLE FOR IT CONTAINS ALCOHOL
IT IS IMPORTANT TO FIX THE SPECIMEN PRIOR TO STAINING  

• Fix with methanol.
• Cover the slide with a 1/20 dilution of Giemsa stain prepared extemporaneously with neutralized water. Allow it to react for 20 to 30 minutes (experience will determine the optimal time).
• Wash up with neutralized water.
• Wipe the slide on the reverse side of the smear.
• Dry: drying oven, hair drier, electric fan  

• Cover the slide with 10 drops of panoptic stain and allow to react for 90 seconds.
• Add the same quantity of hematology adjuvant and allow to react for 90 seconds.
• Rinse with the same adjuvant.
• Wipe the slide on the reverse side of the smear.
• Dry the slide immediately, paying careful attention, for the smear is fragile.